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| Registros recuperados: 109 | |
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| Torres,Alex José Leite; Brígido,Luis Fernando de Macedo; Abrahão,Marcos Herculano Nunes; Angelo,Ana Luiza Dias; Ferreira,Gilcivaldo de Jesus; Coelho,Luana Portes; Ferreira,João Leandro; Jorge,Célia Regina Mayoral Pedroso; Martins Netto,Eduardo; Brites,Carlos. |
| Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a flow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identified by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identification of X4 and R5 tropic viruses was attempted by geno2pheno... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Tropism; Flow cytometry; Geno2pheno. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200163 |
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| Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine. |
| Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf |
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| Rego,E.M.; Garcia,A.B.; Carneiro,J.J.; Falcão,R.P.. |
| The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute lymphoblastic leukemia; Lymphocytes; Quantitative fluorescence cytometry; Flow cytometry; Age-related changes. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200005 |
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| Almeida,AF; Castro,MCAB; Oliveira,AP; Souza,MA; Pereira,VRA. |
| Leishmania infections induce a specific activation of host immunological response, particularly characterized by T cell expansion. Studies indicate the importance of the balance between CD4+ and CD8+ T cells, in which the first ones would have their number reduced during the healing process. Meanwhile, CD25+ T cells have been associated with the suppression of the immune response. Since the immune response has an essential role in both healing and progression of diseases, this study aimed to identify the percentage of CD3+, CD4+, CD8+, CD16+ and CD25+ T cells in the peripheral blood of patients afflicted with American cutaneous leishmaniasis (ACL) - before and after treatment - and healthy controls. Peripheral blood was collected and transferred to... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: American tegumentary leishmaniasis; Cellular immune response; Flow cytometry; Leishmania (Viannia) braziliensis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200016 |
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| Stachowski-haberkorn, Sabine; Becker, Beatriz; Marie, Dominique; Haberkorn, Hansy; Coroller, Louis; De La Broise, Denis. |
| The effects of the herbicide Roundup (R) (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6 m depth, and 10% of their water content was changed every other day. The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 165 and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1 mu g L-1 (ANOSIM,p=0.055; R=0.53), and 10 mu g L-1 (ANOSIM,... |
| Tipo: Text |
Palavras-chave: Microcosm; Marine microbial communities; Glyphosate; TTGE; Flow cytometry. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00088/19887/17546.pdf |
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| Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline; Miner, Philippe; Suquet, Marc; Lambert, Christophe; Soudant, Philippe. |
| The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom; Sperm quality; Cellular parameters; Flow cytometry; Pacific oyster; Crassostrea gigas; Alexandrium catenella. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26853/24982.pdf |
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| Thomas,Angel Treasa; Rao,Josyula Venkata; Subrahmanyam,Volety Mallikarjuna; Chandrashekhar,Hariharapura Raghu; Maliyakkal,Naseer; Kisan,Tukaram Kedar; Joseph,Alex; Udupa,Nayanabhirama. |
| Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticancer; Cytotoxicity; Flow cytometry; Microbial extracts; MTT assay. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200009 |
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| Lambert, Christophe; Soudant, Philippe; Jegaden, Marine; Delaporte, Maryse; Labreuche, Yannick; Moal, Jeanne; Samain, Jean-francois. |
| Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types... |
| Tipo: Text |
Palavras-chave: NO synthase; NADPH oxidase; Flow cytometry; Reactive nitrogen intermediate RNI; Reactive oxygen intermediate ROI; Hemocytes; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3057.pdf |
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| Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
| Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf |
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| Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle. |
| Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that... |
| Tipo: Text |
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf |
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| Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle. |
| Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf |
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| Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry. |
| As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf |
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| Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J.. |
| The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016 |
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| Registros recuperados: 109 | |
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